A Human Case of Hepatic Fascioliasis Accompanied by Egg Granulomas in Common Bile Duct Lymph Node

A 32-year-old housewife who resides in Taegu was admitted in Yeungman University Hospital due to right upper quadrant abdminal pain of 2 mounths'duration. An abdomical CT and ultrasonography revealed a relatively well demarcated low density mass in the right lobe of liver. Right hepatic lobectomy was performed on the clinical impression of hepatoma. On the light microscopic study, the lobulated liver mass showed extensive central necrosis and fibrosis, with large numbers of pseudotubercles therein. The pseudotubercles have distorted helminthic eggs frequently. The submitted common bile duct lymph node also showed a few pseudotubercles. The eggs recovered from the tissue homogenate measured 140~152 micrometer by 75~85 micrometer in size and were unembryonated and light yellow to brown. The eggs were determined as those of Fasciola species. We reported the present case as 11th one of human fascioliasis in korea.

Intensity of infection and development of adult Clonorchis sinensis in hamsters

In an attempt to determine the susceptibility of inbred golden hamsters(Mesocricetus auratus) to the experimental infection with Clonorchis sinensis, twenty-five hamsters were divided into 5 groups and administered orally 5, 10, 20, 30 or 50 metacercariae each. The hamsters were killed on the 45th day after infection. The adult flukes were recovered from all hamsters. The overall recovery rate for the fluke was 57.9 percent with the range of 48.4 percent to 92.0 percent. As the number of the metacercariae given increased, a proportionate decrease in the recovery rate was found. The prepatent period of the fluke in the hamster varied from 16 to 17 days, with the average of 16 days. No significant differences in the development of the flukes were observed. Egg production expressed by EPG, increased step by step as the time elapsed. However, a tendency of decreasing egg laying capacity was observed with the increase of worm burden. It is suggested that the hamster is a suitable final host of C. sinensis.

Passive transfer of immunity against Clonorchis sinensis by peritoneal exudate cells in mice

This study was undertaken to evaluate the role of peritoneal exudate cells in the transfer of immunity against the liver fluke, Clonorchis sinensis in the inbred BALB/c mice. Ten donor mice were divided into 2 groups. One group consisted of 5 mice was infected orally with 20 metacercariae of C. sinensis, and the other group was injected intraperitoneally with 20 excysted larvae. Thirty days after immunization, the peritoneal exudate cells were obtained from the donor mice. Twenty recipient mice were divided into 4 equal groups for the purpose of primary immunization. The mice of Group I were injected intraperitoneally with 2 x 10(6) peritoneal exudate cells of the donor mice infected orally, those of Group III were injected intraperitoneally with 2 x 10(6) peritoneal exudate cells of the donor mice injected intraperitoneally. Those of Group II were injected orally with 20 metacercariae of C. sinensis. The group IV mice served as controls. Four days after the primary immunization all recipient mice were challenged orally with 20 metacercariae of C. sinensis, and then killed 30 days after the challenging infection. When the peritoneal exudate cells were injected into the recipient mice, pronounced reduction in eggs per gram of the feces was found in the mice of Group I and Group II, but no reduction in those of Group III. In the worm burdens of C. sinensis, the number of flukes found in the mice of Group II was only significantly less than those in the control group(IV). In addition the number of plaque forming cells per spleen in the mice of Group II was found larger than those in Group I. It is likely that donor peritoneal exudate cells transferred to the recipients might result in the production of relative immunity.

Epidemiological studies on malayan filariasis in an inland area in Kyungpook, Korea 3. Ecological survey of vector mosquitoes of Brugia malayi

The species composition, population density, and seasonal prevalence of vector mosquitoes in an inland area of Kyungpook province were studied, based on light trap and human bait trap collections, and the recent patterns of infestation for infective larvae of Brugia malayi in these vector hosts were investigated from May to November in 1985. Nine species in four genera of mosquitoes were collected by light trap, human bait trap, and/or by nets. Anopheles sinensis Wiedemann was the most abundant species collected by light traps during this year. Culex tritaeniorhychus Giles was the second abundant species, and Aedes vexans nipponii(Theobald) ranked third in total abundance. The earlist time when A. sinensis were found was the middle of May. At that time the temperature ranged from 14.3 C to 22.8 C and the humidity 53-90 per cent. The month of highest average nightly catch was July, when the temperature was betweeen 21.5 C and 30.6 C and the humidity 72-91 per cent. The peak time of biting activity of mosquitoes was different in each month, i.e. between 22:00-23:00 in July, and 20:00-21:00 hours in September, when the temperature was between 24.3 C and 26.5 C and the humidity 73-88 per cent in the field. While infective larvae of B. malayi were reported to have been found in one species of mosquito in 1975, no larvae were found in any species collected in this survey.

Infestation of larval trematodes from fresh-water fish and brackish-water fish in river Taechong, Kyungpook province, Korea

: A study of infestation patterns for larval trematodes from fresh-water fish and brackish-water fish was carried out during the period from June to October in 1982. They were collected by netting, fishing with rod and line, and using the bait of crushed oil cake in a transparent plastic bowls at the three localities, Daebon-dong, Kugil-dong, and Hoam-dong in the river Taechong, Kyungpook Province, Korea. Five species of fresh-water fish, the crussian carp, Carassius carassius Linnaeus, the bullhead, Coreobagrus brevicorpus Mori, the fat minnow, Moroco oxycephalus (Bleeker), the flat bitterling, Paracheilognathus rhombea(Temminck et Schlegel), and the catfish, Parasilurus asotus (Linnaeus), and two kinds of brackish-water fish, the grey mullet, Mugil cephalus (Linnaeus) and the sweetfish, Plecoglossus altivelis (Temminck et Schlegel) were examined. Four species of the metacercariae, Exorchis oviformis, Metacercari hasegawai, Metorchis orientalis and undetermined larvae were found. Of these, the encysted larvae of the human intestinal fluke, Metagonimus yokogawai, were found from three kinds of fresh-water fish, the crussian carp, the bullhead and the fat minnow and one species of brackish-water fish, the sweetfish and the infestation rates for the larvae varied appreciably from fish to fish. The intensity of infestation with the Metagonimus larvae in the three species of fresh-water fish as low, and the average number of the larvae per gram of flesh varied from 0.5 to 0.2. By comparison, the brackish-water fish was more heavily infested and the mean number of cysts per gram of flesh in Plecoglossus altivelis was 109.2. It was found that infection with Metagonimus yokogawai is caused by consuming raw fresh-water fish and brackish-water fish caught in the river Taechong, Kyungpook Province, Korea.

Recent demonstration of helminth eggs and larvae from vegetable cultivating soil

A survey on soil samples collected from the vegetable gardens and vinyl houses in the vicinity of Taegu was conducted for the discovery of helminth eggs and larvae from October 1981 to September 1982. The samples collected were examined utilizing the "Commonly Employed" and Kamiya's techniques. The details of comparative features of larval nematodes are taken from the handbook of parasitology by Matsubasyhi et al. (1965). The results were compared with the data obtained from soil samples collected at the same area by Lee(1976). Five kinds of helminth eggs, ascarid, hookworm, liver fluke, Trichostrongylidae, and whipworm and undetermined eggs were found. Of these, the incidence of ascarid eggs was found to be the highest, 70.5 per cent, followed by whipworm and hookworm eggs, 9.9 and 9.2 per cent, respectively. The average number of ascarid eggs per 100 grams of soil was higher in the vinyl houses, compared with the number in the vegetable gardens than in the vinyl houses. By contrast, the number of hookworm eggs was relatively high in the vegetable gardens. In the monthly incidences of helminth eggs, ascarid eggs were found every month, continuing fairly high incidences all the year round. However, hookworm eggs was found for 8 months from February and September, with low monthly incidences between 4.0 and 22.2 per cent, and Trichostrongylidae eggs were found for 6 months from April to September, with lower incidences than those of hookworm. Three kinds of larval nematodes, the filariform and rhabditoid larvae of hookworm, Trichostrongylidae, and undetermined larvae were found in the soil of farms, but the incidences of larvae were quite low. The average number of larvae per 100 grams of soil was 0.05. When the number of eggs per 100 grams of the soil in 1976 were compared with 1982, pronounced reduction of the number was encountered in all helminth eggs. However, the incidences and the average number of larvae per 100 grams of the soil were so low that it was impossible to compare the data between 1976 and 1982.

Larval trematodes from Semisulcospira snails in Kyungpook Province, Korea

In an attempt to determine the incidence of the cercarial larvae of trematodes, Semisulcospira snails collected in Judong stream, an endemic area of Paragonimus westermani, in Kyungpook Province, Korea, were examined from 1965 to 1980. The infestation rate for cercariae in the snails was 5.4 per cent, but in 1970 dropped to 4.0 per cent and remained at the same level, 4.2 per cent, in 1975. It then decreased to 3.5 per cent in 1980. Seven species of cercariae, Cercaria incerta(Kobayashi, 1992) Faust, 1924, Cercaria nipponensis(Ando, 1915) Faust, 1924, Cercaria yoshidae(Osafune, 1898) Cort et Nichols, 1920, Centrocestus armatus(Tanabe, 1922) Yamaguti, 1934, Metagonimus species, Notocotylus magniovatus Yamaguti, 1934, and Paragonimus westermani(Kerbert, 1878), and five kinds of undeterminced cercariae were found. Of these, the most freqently emerged cercaria was Metagonimus species, folllowed by Notocotylus magniovatus. The least freqently emerged was Paragonimus westermani, with the rate of 0.04 per cent. The liberation rates in the five kinds of undetermined species varied from 0.04 to 0.15 per cent. In the monthly liberaton rates for cercariae, the highest rate was encountered in May, and decreased in the period from June to November. It then somewhat increased again in December. There was no correlation in the liberation rate for cercariae and length of snails. The results of this study indicate that the infestation of Semisulcospira snails with the cercariae of trematodes has decreased over the fifteen year period from 1965 to 1980.

Infestation with larval trematodes from fresh-water fish in natural and fish breeding ponds

The present study was undertaken to determine the pattern of infestation with the encysted larvae of digenetic trematodes in freshwater fish collected in both the natural and fish breeding ponds in Kyungpook Province, Korea. The fish collected in the two natural ponds, Nass-chil, and Sa-il, and three fish breeding ponds, Deok-dong, Mo-gok, and Seongryu-tunnel, were examined from July 1980 to August 1981. The data were compared with the results obtained from freshwater fish caught in the river Kumho by Hwang and Choi(1980). Ten species of fish caught in the ponds were examined for the presence of the larval trematodes. Seven species of the larvae, Clonorchis sinensis, Cyathocotyle orientalis, Echinochasmus species, Exorchis oviformis, Metacercaria hasegawai, Metagonimus yokogawai, and Metorchis orientails and two kinds of undetermined cyst A and B were found. The infestitation rate for Clonorchis sinensis metacercaria in the Korean shiner, Gnathopogon atromaculatus collected in the natural ponds was higher than the rate in the river Kumho by Hwang and Choi(1980), but lower in the fish collected in the breeding ponds. By contrast, the infestation rate in the flat bitterling, Paracheilognathus rhombea in ponds was higher, while there was no change of the rate in the southern top-mouthed minnow, Pseudorasbora parva. The intensity of infestation, expressed as the average number of Clonorchis sinensis metacercaria per gram of flesh, were lower than in the bullhead, Coreobagrus brevicorpus, and Gnathopogon atromaculatus in the ponds in 1981. The intensity was higher in Paracheilognathus rhombea, but no fluctuation in the intensity of infestation in Pseudorasbora parva was observed. The infestation rates and densities for the encysted larvae of digenetic trematodes except for Clonorchis sinensis varied from fish to fish. However, it was found that the rate and intensity of the larval trematodes from all the fish in the ponds was lower than from those in the rivers in Kyungpook Province, Korea.

Epidemiological study of Paragonimus westermani in Wiseong County, Kyungpook Province, Korea

This survey was undertaken to determine the recent prevalence of lung fluke, Paragonimus westermani, in the three localities of Wiseong county, Kyungpook Province, Korea and to compare the prevalence among the residents in the same areas between 1924 and 1980. The three localities, Ku-seong, Sam-chun, and Um-ji villages (dong) have been known as the endemic areas of Paragonimus westermani. The surveys were made on the general population. They were examined using intradermal test with Paragonimus westermani antigen, and the diagnosis of paragonimiasis was made depending on the positive intradermal reaction. The sputum examination could not be performed beacause the residents were inaccessible. The prevalence of Paragonimus westermani among the residents in the three communities varied from 1.4 percent to 7.9 per cent, with an average of 4.0 per cent. Whereas, the average reported by Ichinomiya in 1924 was 4.7 percent. There are no significant differences in the sex and age specific rates of infection between 1924 and 1980. With the abundant density of Semisulcospira snails and crayfish in Sam-chun stream and the high rate of infection with Paragonimus westermani among the residents in the village, it is found that Sam-chun village, one of the three localitied in Wiseong county, where Ichinomiya (1924) surveyed, was still the endemic focus of Paragonimus westermani.

Demonstration of Entamoeba histolytica cyst from urban and rural school children

School children between 6 to 12 years of age were studied for the presence of Entamoeba histolytica cysts in a period of 6 months, form March to August 1980. Fecal specimens collected form urban and rural children were examined first by the Lugol stained technique and then by the Faust's iron alum hematoxylin method. A total of 731 specimens were examined. Of these 26.9 per cent, 197 specimens, harbored the cysts. The demonstration rate for the cysts by age group varied from 19.6 to 43.5 per cent. The demonstration rate in boys was 24.4 per cent and in girls 29.8 per cent. Similarily, the rate in urban and rural area was 32.4 and 24.1 per cent, respectively. It was found in the present study that there were no significant differences in the age and sex rates of demonstration between urban and rural children but there was a predominance of the small race of Entamoeba histolytica over the large race.

Seasonal variation of snail population of Parafossarulus manchouricus and larval trematode infection in river Kumho, Kyungpook province, Korea

Studies were conducted to determine the ecology of the snail, Parafossarulus manchouricus, the seasonal variation of the snail population, and the infestation rates of these snails with the cercariae of digenetic trematodes, in the snail habitat of river Kumho, in Dongchon of Taegu city, in Kyungpook Province, Korea, from 1975 to 1979. The earliest time the snails were found was early in April every year. At that time the water temperature ranged from 13 to 14 degrees C. The population density of snails ranged from 2 to 8 per square meter. The highest population density of snails was encountered in late June and the water temperature was between 24 and 26 degrees C. The snails disappeared in early and mid-November, when the water temperature dropped to 10-13 degrees C. The average snail population in June was 115.9 per square meter in 1975 but in 1976 dropped abruptly to 30.5 and remained at approximately the same level in 1977. It then decreased at considerably lower rates, with the average of 18.7 in 1978 and 14.5 in 1979. The snails collected in the habitat were examined for the presence of cercariae of digenetic trematodes. Of these, four species, Clonorchis sinensis, Cyathocotyle orientalis, Exorchis oviformis, and Loxogenes liberum were found. The most frequently liberated cercaria was L. liberum 43.3 per cent, followed by E. oviformis 6.2 per cent and C. orientalis 1.5 per cent. The least frequently liberated was C. sinensis 0.1 per cent. In the monthly liberation rates for larval trematodes, the higher rates were observed in June, July and August every year, and the cercarial rates for Clonorchis sinensis, Cyathocotyle orientails and Exorchis oviformis demonstrate a fairly consistently fluctuating downward trend over the five-year period, except for Loxogenes liberum. Summarizing the results, this study indicated that the population density of snails in the habitat and the infection of the snail with the cercariae of digenetic trematodes has decreased over the five-year period from 1975 to 1979.

Demonstration of Metagonimus yokogawai metacercariae from Plecoglossus altivelis in river Ahnseong, Kyungpook province, Korea

There are a number of eating-houses in the basin of river Ahnseong that sell rawsweetfish, Plecoglossus altivelis, to local residents and visitors. These facts called attention to the possibility of wide spread of metagonimiasis in that area. In an attempt to determine whether the metacercaria of Metagonimus yokogawai does present in the sweetfish caught in the river Ahnseong, a total of 77 fish were examined in the period from April to September 1978. All the fish were infected with the encysted larvae. The average number of larvae persweetfish was 1,815, of which the metacercaria rate for the fluke in the flesh was 56.9 per cent and in the scale, 30.5 per cent. These results indicate that all the sweetfish in the river Ahnseong harbour the metacercaria of Metagonimus yokogawai.

Prevalence of Clonorchis sinensis in vicinity of Seongju, Kyungpook Province, Korea

An epidemiological survey conducted from March 1977 to October 1978, revealed that in the river Taeka running through Seong-ju county, Kyungpook Province, one habitat of the snail, Parafossarulus manchouricus, was found. The snail population in the habitat was very low, and no Clonorchis sinensis cercaria was found in the snails examined. Seven species of fresh-water fish caught were examined for the presence of the metacercaria of Clonorchis sinensis. Of these, 3 species of fish, the Korean shiner, Gnathopogon atromaculatus, the gudgeon, Pseudogobio esocinus, and the striped shiner, Pungtungia herzi harbored the encysted larvae. The rate and intensity of infection with the metacercaria in the fish was, on the whole, low. The prevalence of Clonorchis sinensis among the residents in Seong-ju county was also low. The overall infection rate for the fluke in 1,012 residents was found to be 13.4 per cent. The infection rate for Clonorchis sinensis in males and females was 17.1 and 9.2 per cent, respectively. This difference was statistically significant ( t > 2). It is found that Clonorchis sinensis is less prevalent in Seong-ju county than in the other counties of Kyungpook Province, Korea.

Endemic foci of Paragonimus westermani in Chilgok, Kyungpook Province, Korea

By performing the county-wide surveys of Paragonimus westermani infection in several areas in the Chilgok county, Kyungpook Province, three endemic foci of Paragonimus westermani were found. The foci found in this survey were Haksan first dong, Haksan second dong, and Settom in Kumhwa village, all in Kasan myun, Chilgok county. In the three communities, the positive rates for the fluke among the residents by the intradermal test with Paragonimus antigen varied from 22.3 per cent to 25.0 per cent; school children from these areas showed infection rates above 50 per cent. The rate in males and females was 23.9 and 23.0 per cent, respectively. This difference was not significant. The presence and number of Semisulcospira snails and Astacus crayfish, the intermediate hosts of Paragonimus westermani in the stream communities and the high infection rates for the fluke in the residents were the supporting factors in determining the foci.

Metacercarial density of Metagonimus yokogawai in Plecoglossus altivelis in Kyungpook province, Korea

In order to determine the intensity of infection with the encysted larvae of Metagonimus yokogawai among Plecoglossus altivelis, sweetfish, caught at the rivers in Kyungpook Province, Korea were examined in the period from July to October, 1976. The data were compared with the results obtained from Tribolodon hakonensis, the sea-run dace, caught at the river Hyungsan in the Province. A total of 128 Plecoglossus altivelis were examined. All the fish were infected with the metacercaria of Metagonimus yokogawai. The metacercarial rate for the fluke in the flesh was higher than the rates in the fins and scales. The average number of larvae per sweetfish was 4,333, compared with the average of 1,541 from the sea-run dace. It was found that the highest number of encysted larvae was found in the flesh of sweetfish, whereas it was encountered on the scales of sea-run dace. In the distribution pattern of the cysts, there was no relationship between the size of fish and the intensity of infection.

Clonorchis sinensis in Kyungpook Province, Korea 3. Changing pattern of Clonorchis sinensis infection among inhabitants

This study was undertaken to evaluate the effects of health education and anti-Clonorchis campaign conducted in Kyungpook Province and to compare the prevalence of Clonorchis sinensis among the residents in the same areas for the period 1964-1976. Both surveys were made on all age groups of residents ranging from 0 to over 60 years of age. They were examined using primarily intradermal tests with Clonorchis and Paragonimus antigens and followed by stool examinations. The definitive diagnosis of clonorchiasis was made on the recovery of Clonorchis eggs from the stools. A marked reduction in the prevalence was encountered in the youngest age group, but there is no significant difference in the older age groups. The overall prevalence rate for clonorchiasis was reduced from 27.7 to 19.6 per cent in a period of 10 years. It is found that clonorchiasis is less prevalent in Kyungpook Province than it was a decade ago and it continued to decline.

Clonorchis sinensis in Kyungpook province, Korea 2. Demonstration of metacercaria of Clonorchis sinensis from fresh-water fish

From March 1973 to September 1974, fresh-water fishes were collected in rivers in Kyungpook Province, Korea, using three collecting technics; throwing a cast-net, fishing and using a glass bowl containing crushed oil-cake as a bait. Twenty-one species of the fish caught in the rivers in Kyungpook Province were examined for the presence of the metacercaria of Clonorchis sinensis. Of these, 10 speciec of the fish harbored the encysted larvae. The most frequently infected one was Pseudorasbora parva 92.3 per cent, followed by Pseudogobio esocinus 90.4 per cent, Sarcocheilichthys sinensis 87.7, Hemibarbus labeo 87.2, and Pungtungia herzi 60.8 per cent, in decreasing order. The less frequently infected were Erythroculter erythropterus 13.0, Acanthorhodeus taenianalis 7.7, Paracheilognathus rhombea 5.9 and Acheilognathus limbata 4.2 per cent. In the intensity of infection with the larvae, Pseudorasbora parva was most heavily infected and the mean number of metacercaria per ml of flesh was 51.4, Pungtungia herzi, Gnathopogon atromaculatus, Pseudogobio esocinus, Sarcocheilichthys sinensis and Homibarbus labeo were infected intermediately (Their mean number varied 10.3 to 19.5). Whereas, Paracheilognathus rhombea, Acheilognathus limbata and Acanthorbodeus taenianalis were infected with a few cysts.

Clonorchis sinensis in Kyungpook Province, Korea 1. Distribution and demonstration of the cercaria of Clonorchis sinensis from snail, Parafossarulus manchouricus Bourgigant

Studies were conducted in rivers in Kyungpook Province, Korea from July 1970 to September 1974 to determine the stations where Parafossarulus manchouricus, the intermediate snail host of Clonorchis sinensis, are abundant, and the infection rates of these snails with the cercaria of Clonorchis sinensis. Snail collections were made during four consecutive summers(July to September in each year). Ten snail habitats were found. The population density of the snails per square meter of the river bottom ranged from 25 to 250. The highest population of snails was encountered at the Dong-chon station. Cercaria studies by means of the immersing and crushing technics indicated surprisingly low rate of Clonorchis sinensis infection among the snails examined. The proportion of infected snails in Kyungpook Province was 0.8 per thousand of all snails examined. Results indicated that the Parafossarulus manchouricus exists in limited areas within the rivers under study and the infection of the snail with the cercaria of Clonorchis sinensis is very low.

Incorporation of exogenous D-glucose-1-(14)C and D-manose-1-(14)C into glycogen and lactate excreted by Angiostrongylus cantonensis in vitro

The amount of labelled glucose incorporated into glycogen is 6 times as much as that of labelled mannose by the female worms. They excreted lactate of labelled glucose-origin 3-4 times as much as that of labelled mannose-origin. The amount of labelled glucose incorprated in worm bodies is about 3 times as much as that of labelled mannose. Male worms absorbed a half amount of glucose by female worms. Paralelled with radioactivity studies mentioned above, chemical determinations of sugars, lactate, and glycogen were performed on the media prior and posterior to incubation and the worms. The results obtained from chemical determinations fairly agreed with those on lactate production and sugar consumption from radioactivity studies, but not on glycogen content of worms after incubation. Chemical determination shows no glycogen synthesis in worms incubated in mannose media while the radioactivity of labelled mannose was detected, though it is very low in activity, from glycogen of worms in the media. Coupled with previously reported findings, results from the present study show mannose to be far less significant in the carbohydrate metabolism of Angiostrongylus cantonensis as compared with glucose.

Newly found endemic foci of Clonorchis sinensis in Kyungpook Province, Korea

Nishimura in 1943 indicated for the first time that a serious endemic focus of Clonorchis sinensis is located in the vicinity of Yeong-cheon(Kyungpook Province, Korea). Later, numerous surveys of this fluke in the Province have been made, but no additional report on the endemic focus of the fluke has been available. The overall percentage of Clonorchis sinensis infections, based on the discovery of eggs by the formalin-ether sedimentation technique, was found to be 54.8 percent from 851 residents in Nakjeon ri (village), Danmil myun, Uiseong gun (county), Kyungpook Province and no significant difference was observed in the sex specific rate of the individuals infected. There is a high incidence of infection with Clonorchis sinensis. The infection rate in the age group from 0 to 4 years was 14.6 per cent, 5 to 9 years was 35.7 per cent and 10 to 14 years was 51.7 per cent. In the worn burdens for residents, the average number of egg-counts was 41,800. It is found that a serious endemic focus of Clonorchis sinensis exists in the vicinity of Nakjeon village.